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1、热烈祝贺钟山生命科学园更名,园区环境大幅改善。

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文献专区
分子生物
* Identification and validation of reference genes for quantitative real-time PCR studies in long yellow daylily, Hemerocallis citrina Borani. Feifan Hou et al.PLoS One. 2017; 12(3): e0174933. doi: 10.1371/journal.pone.0174933
* KR3和Hs1基因聚合对大豆花叶病毒病抗性的影响 Yu XQ. Zhejiang University, 2016.
* 家蚕c-Myc基因的全长cDNA克隆及促细胞增殖功能初探 Xu Hanfu et al.State Key Laboratory of Silkworm Genome Biology,Southwest University; Science of Sericulture,2015 February.
* Isolation and characterization of a proteinaceous α-amylase inhibitor AAI-CC5 from Streptomyces sp. CC5, and its gene cloning and expression.Sun, Z., Lu, W., Liu, P. et al. Antonie van Leeuwenhoek (2015) 107: 345. doi:10.1007/s10482-014-0333-y.
* Molecular characterization and detection of a spontaneous mutation conferring imidazolinone resistance in rapeseed and its application in hybrid rapeseed production. Hu, M., Pu, H., Kong, L. et al. Mol Breeding (2015) 35: 46. doi:10.1007/s11032-015-0227-3.
* 猪带绦虫双歧杆菌表达系统pGEX-TSOL18/B.longum的构建及鉴定 Zhou Bi-ying et al. Department of Parasitology,Zunyi Medical College. Chinese Journal of Parasitology and Parasitic Diseases. 2014 March.
* 家蚕卵黄原蛋白受体BmVgR的体外表达 Luo Juan et al, Scientia Agricultura Sinica, 2014 19.
* Deng Y et al.The role of miR-31-modified adipose tissue-derived stem cells in repairing rat critical-sized calvarial defects.2013 Sep;34(28):6717-28. doi: 10.1016/j.biomaterials.2013.05.042. Epub 2013 Jun 13.
* Deng Y et al. Effects of a miR-31, Runx2, and Satb2 regulatory loop on the osteogenic differentiation of bone mesenchymal stem cells.2013 Aug 15;22(16):2278-86. doi: 10.1089/scd.2012.0686. Epub 2013 Apr 27.
 
蛋白表达
* Identification and validation of reference genes for quantitative real-time PCR studies in long yellow daylily, Hemerocallis citrina Borani. Feifan hou.PLoS One. 2017, doi: 10.1371/journal.pone.0174933
* Transcriptional profiling analysis of Spodoptera litura larvae challenged with Vip3Aa toxin and possible involvement of trypsin in the toxin activation.Feifei Song et.al.Sci Rep. 2016; 6: 23861.doi: 10.1038/srep23861
* Activation of PPARγ by a Natural Flavonoid Modulator, Apigenin Ameliorates Obesity-Related Inflammation Via Regulation of Macrophage Polarization.et al.Xiujing Feng et al.EBioMedicine. 2016 Jul; 9: 61–76.doi:10.1016/j.ebiom.2016.06.017
* Molecular characterisation of the vitellogenin gene (AlVg) and its expression after Apolygus lucorum had fed on different hosts. Sun Y et al. Pest Management Science, Volume 72, Issue 9, 22 JAN 2016.
* Hydrogen Sulfide Regulates Krüppel‐Like Factor 5 Transcription Activity via Specificity Protein 1 S‐Sulfhydration at Cys664 to Prevent Myocardial Hypertrophy.Guoliang Meng et al. J Am Heart Assoc. 2016 Sep; 5(9): e004160.doi: 10.1161/JAHA.116.004160
* Identification and Comparative Analysis of CBS Domain-Containing Proteins in Soybean (Glycine max) and the Primary Function of GmCBS21 in Enhanced Tolerance to Low Nitrogen Stress.Qingnan Hao et al.Int J Mol Sci. 2016 May; 17(5): 620.doi: 10.3390/ijms17050620
* Expression and prognostic value of E-cadherin in laryngeal cancer. Xiaoyun Qian et al, Acta Oto-Laryngologica Volume 136, 2016 - Issue 7.
* Preparation and characterization of a stable BHK-21 cell line constitutively expressing the Schmallenberg virus nucleocapsid protein. Yongning Zhang et al. Molecular and Cellular Probes Volume 29, Issue 4, August 2015, 244–253
* Recombinant rat CC16 protein inhibits LPS-induced MMP-9 expression via NF-κB pathway in rat tracheal epithelial cells. Min Pang et al.Exp Biol Med (Maywood). 2015 Oct; 240(10): 1266–1278.doi: 10.1177/1535370215570202
* ROS activates JNK-mediated autophagy to counteract apoptosis in mouse mesenchymal stem cells in vitro.Guanyu Liu et.al.Acta Pharmacol Sin. 2015 Dec; 36(12): 1473–1479. doi: 10.1038/aps.2015.101
* ER Stress Mediates TiAl6V4 Particle-Induced Peri-Implant Osteolysis by Promoting RANKL Expression in Fibroblasts.Zhenheng Wang.et al.PLoS One. 2015; 10(9)doi: 10.1371/journal.pone.0137774.
* 稳定表达施马伦贝格病毒核衣壳蛋白的BHK-21细胞系的建立 Zhang YN et al. Shandong Agricultural University, Chinese Journal of Preventive Veterinary Medicine, 2015, 06.
* Characterizing heat shock protein 90 gene of Apolygus lucorum (Meyer-Dür) and its expression in response to different temperature and pesticide stresses. Yang Sun. et.al.Cell Stress Chaperones. 2014 Sep; 19(5): 725–739.doi: 10.1007/s12192-014-0500-0
* Cloning and prokaryotic expression of GmNAC8 gene isolated from soybean.LIU Xiao-qing et al. Jiangsu Journal of Agricultural Sciences,2014, 04.
* Expression and purification of soluble human APRIL in Ecoli using ELP-SUMO tag. Zhang J. et al.Protein Expr Purif. 2014 Mar;95:177-81. doi: 10.1016/j.pep.2013.12.013. Epub 2014 Jan 10.
* 罗非鱼无乳链球菌C5a肽酶(ScpB)的原核表达及其免疫原性 Li QY et al. Shanghai Ocean University,Journal of Fishery Sciences of China, 2014, 01.
* 中国美利奴绵羊磷脂酶C zeta基因表达载体的构建及原核表达 MU Er zha ti·A le teng bie ke et al. Shihezi University, Progress in Veterinary Medicine, 2014,12.
* 苏云金芽胞杆菌WB5中营养期杀虫蛋白3Aa基因(vip3Aa)的克隆、表达与纯化 Chen FB et al.Fujian Agriculture and Forestry University,Journal of Agricultural Biotechnology, 2014, 11.
* 油菜乙酰羟基酸合酶基因BnAHAS1的克隆及其重组蛋白质的原核表达 Hu ML et al. 江苏农业学报, 2014, 05.
* Characterizing heat shock protein 90 gene of Apolygus lucorum (Meyer-Dür) and its expression in response to different temperature and pesticide stresses. Sun Y. Cell Stress Chaperones. 2014 Sep;19(5):725-39. doi: 10.1007/s12192-014-0500-0. Epub 2014 Mar 13.
* 间日疟原虫醛缩酶的克隆、表达与初步鉴定 Gao Ying et al. Nanyang Central Hospital, Journal of Bengbu Medical College,2013, 07.
* 10-HDA对意蜂幼虫发育影响及中蜂Royalactin mRNA水平和原核表达分析 Zeng ZJ. Jiangxi Agricultural University, 2013.
* Resilin-R5融合蛋白表达纯化及其材料性能研究 Mao ZZ, Huazhong Agricultural University, 2013 .
* 罗非鱼3种C型溶菌酶重组蛋白的制备及与几种鱼虾溶菌酶溶菌谱的比较 Qu Lan et al.Shanghai Ocean University, Biotechnology Bulletin, 2012, November.
* Production of bioactive human beta-defensin-4 in Ecoli using SUMO fusion partner. Li JF. et al. Protein J. 2011 Jul;29(5):314-9. doi: 10.1007/s10930-010-9254-4.
 
抗体制备
* miR-223 increases gallbladder cancer cell sensitivity to docetaxel by downregulating STMN1.Wei Lu et al.Oncotarget. 2016 Sep 20; 7(38): doi: 10.18632/oncotarget.11634
* Xin Wang, Ping Zhao, Yi Li, Qiying Yi, Sanyuan Ma, Kang Xie, Huifang Chen, Qingyou Xia*. Modifying the mechanical properties of silk fiber by genetically disrupting the ionic environment for silk formation. Biomacromolecules, 2015.
* 金属离子和离子转运蛋白在蚕丝成纤过程中的功能研究 Wang X. Southwest University,2016.
* 白蜡虫脂酰辅酶A还原酶多克隆抗体的制备 Hu YH et al. Forest Research, 2014, 01.
* 草鱼呼肠孤病毒HZ08株S10编码蛋白多克隆抗体制备及其特性分析 Li YG et al. Journal of Fisheries of China, 2014, 03.
* 异常凝血酶DCP基因克隆表达、纯化及单克隆抗体制备与鉴定 Shen P et al. World Chinese Journal of Digestology, 35.
* 新型Resilin-R5融合蛋白的大肠杆菌表达和纯化 Mao ZZ. Acta Agriculturae Zhejiangensis, 2014, 01.
* 罗非鱼无乳链球菌LrrG蛋白的原核表达及免疫原性分析 Chen Xue et al. Journal of Fisheries of Chinsa, 2014, 05.
* 猪带绦虫TSO45W-4B基因的克隆、表达和抗体制备 Zhou BY et al. Chinese Journal of Parasitology and Parasitic Diseases, 2013, 05.
* 猪带绦虫TSOL18基因的表达、纯化和兔抗血清的制备 Zhou BY et al. Chinese Journal of Zoonoses, 2013, 10.
Simultaneous Detection of Fenitrothion and Chlorpyrifos-Methyl with a Photonic Suspension Array.Xuan Wang et al.PLoS One. 2013; 8(6): e66703. doi: 10.1371/journal.pone.0066703
* 罗非鱼无乳链球菌C5a肽酶的原核表达及其免疫原性研究 Li QY. Shanghai Ocean University, 2013.
* 小鼠肝炎病毒N蛋白单克隆抗体的制备及鉴定 Zhou J et al. Chinese Journal of Cellular and Molecular Immunology, 2012, 08.
 
免疫检测
* Luteoloside Acts as 3C Protease Inhibitor of Enterovirus 71 In Vitro.Zeyu Cao et al.PLoS One. 2016; 11(2): e0148693.doi: 10.1371/journal.pone.0148693
* 禾谷镰刀菌几丁质合成酶的功能研究 Zhang XP. Zhejiang University, 2016. (Southern Blot)
* Recombinant rat CC16 protein inhibits LPS-induced MMP-9 expression via NF-κB pathway in rat tracheal epithelial cells.Min Pang et al.Exp Biol Med (Maywood). 2015 Oct; 240(10): 1266–1278.doi: 10.1177/1535370215570202
* Biotic stress-induced expression of mulberry cystatins and identification of cystatin exhibiting stability to silkworm gut proteinases. Liang, J., Wang, Y., Ding, G. et al. Planta (2015) 242: 1139. doi:10.1007/s00425-015-2345-x.
* 醋酸棉酚对人舌鳞癌Tca8113细胞hMLH1蛋白及mRNA表达的影响 Chen WF et al. Journal of Kunming Medical University, 2014, 01.
* 取食不同寄主植物对绿盲蝽热激蛋白AlHSP90表达的影响 Sun Y et al. 棉花学报 2014,02.
* 唐山地区儿童急性中耳炎常见病原菌及药物敏感性分析 Liu JY et al. Tangshan People's Hospital, 2014.
* 家蚕核型多角体病毒egt基因的原核表达及侵染BmE细胞中的EGT蛋白检测 Liu HL el al. Science of Sericulture, 2013, 06.
* 家蚕卵黄原蛋白受体(BmVgR)与配体结合及解离机制的初探 Luo J et al. Southeast University, 2013
* 罗非鱼3种C型溶菌酶重组蛋白的制备及与几种鱼虾溶菌酶溶菌谱的比较 Qu Lan et al. Biotechnology Bulletin, 2012, 11.
 
理化检测
* MoDnm1 Dynamin Mediating Peroxisomal and Mitochondrial Fission in Complex with MoFis1 and MoMdv1 Is Important for Development of Functional Appressorium in Magnaporthe. oryzae. Kaili Zhong el.al. PLoS Pathog. 2016 Aug; 12(8): e1005823.doi: 10.1371/journal.ppat.1005823
* Effect of Removing Superior Spikelets on Grain Filling of Inferior Spikelets in Rice. Cuicui You et al. Front Plant Sci. 2016; 7: 1161. Published online 2016 August 5. doi: 10.3389/fpls.2016.01161

 

技术交流专区
分子生物
 
蛋白表达
M001 质粒DNA 的分离、纯化和鉴定   P001 大肠杆菌感受态细胞的制备和转化
M002 DNA 酶切及凝胶电泳   P002 原核蛋白表达及纯化案例
M003 RNA 的提取和cDNA 合成   P003 毕赤酵母表达及纯化案例
M004 基因组DNA 的提取   P004 昆虫系统表达及纯化案例

 

抗体制备
 
免疫检测
A001 抗体分类及特性和功能   I001 各类免疫球蛋白主要理化特性和生物学特性比较
A002 抗原选择及多肽抗原设计原则   I002 Western Blot检测案例
A003 抗原分类及制备方法   I003 Southern Blot检测案例
A004 多克隆抗体制备案例   I004 实时荧光定量PCR案例

 

理化检测
 
H001 植物激素简介    
H002 理化检测服务    
H003 激素检测案例    
H004 乙烯前体简介